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Cellular trans-membrane signalling mechanisms are among the most highly studied processes in the biological sciences. G-proteins provide transduction components to allow communication between receptors for hormones and neurotransmitters and their effector systems which include enzymes involvedin the generation of intracellular secondary messengers and a variety of ion channels. Signal Transduction: A Practical Approach details the experimental approaches which are used to define the contacts between receptors and G-proteins and between these G-proteins and their effector systems. While
much emphasis in the cellular signalling field remains on the measurement of adenylyl cyclase and of phosphoinositidase C activities, it is now clear that activation of G-protein linked receptors can also cause the regulation of phospholipases of the D and A2 classes. Thus, assays used to measure
the activities of these enzyme systems are detailed in this book. The use of mutagenesis to define functional domains of G-proteins themselves has undergone considerable progress recently and examples of this approach are also included. This volume provides a useful guide to the newcomer to the
subject, as well as allowing workers already in the field to expand the range of approaches in use in their laboratories

Otros libros del autor

• Cell-Cell Interactions
• Cellular Neurobiology
• Cytoskeleton, The
• Gene Targeting
• Gene Transcription
• In Situ Hybridization
• Molecular Neurobiology
• Receptor-Effector Coupling

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